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MB Sample ID: SA331979
| Local Sample ID: | R_cont_3 |
| Subject ID: | SU003179 |
| Subject Type: | Plant |
| Subject Species: | Amaranthus caudatus L. |
| Taxonomy ID: | 3567 |
| Gender: | Not applicable |
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Sample Preparation:
| Sampleprep ID: | SP003185 |
| Sampleprep Summary: | Approximately 10 and 20 mg of ground dry leaf and root material, respectively, were extracted with 1 mL methanol. After vortexing (3000 g, 30 s) and centrifugation (12000 g, 4 °C, 10 min) of the suspensions, the resulted supernatants were collected. The plant material residues were additionally supplemented with 0.1 mL of deionized water. After a following vortex and centrifugation cycle, the obtained supernatants were combined with the first portions. The total extract volume was 1090 μL. Aliquots (30 μL) of the resulted aq. methanolic extracts were freeze-dried under reduced pressure with Labconco CentriVap centrifugal concentrator. The residues were sequentially derivatized with methoxyamine hydrochloride in pyridine, and N-methyl-N-(trimethylsilyl) trifluoroacetamide (MSTFA) according to the established procedure (Leonova et al., 2020, http://dx.doi.org/10.3390/ijms21020567). |
| Processing Storage Conditions: | 4℃ |
| Extract Storage: | -20℃ |
