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MB Sample ID: SA332092
| Local Sample ID: | C2 |
| Subject ID: | SU003183 |
| Subject Type: | Cultured cells |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Genotype Strain: | AGS and AZ-521 |
| Species Group: | AGS and AZ-521 |
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Sample Preparation:
| Sampleprep ID: | SP003189 |
| Sampleprep Summary: | Briefly, cell pellets were normalized by total protein (200 ug) and the corresponding cell supernatants were normalized by volume (200 uL). Metabolites were extracted with methanol/water 80:20. Heavy labeled phenylalanine-D8 and biotin-D2 were added to individual samples prior to protein precipitation. Following overnight incubation at -80°C, precipitated proteins were pelleted by centrifugation at 10,000 rpm for 10 min and metabolite extracts were transferred into two Eppendorf tubes in equal amounts and dried down in vacuo and stored at -80°C. |
| Sampleprep Protocol Filename: | MS_Methods_Cover_VacA_Mutants.pdf |
| Processing Storage Conditions: | -80℃ |
| Extract Storage: | -80℃ |
