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MB Sample ID: SA333597
Local Sample ID: | old_7 |
Subject ID: | SU003219 |
Subject Type: | Human |
Subject Species: | Homo sapiens |
Taxonomy ID: | 9606 |
Age Or Age Range: | Young donors (aged 25 years or younger) and older donors (aged 50 years or older). |
Gender: | Male and female |
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Sample Preparation:
Sampleprep ID: | SP003225 |
Sampleprep Summary: | Briefly, approximately 50 mg of ground heart tissue was weighed into 2 mL Eppendorf tube and subjected to a two-phase extraction protocol involving tissue, ice-cold extraction medium (methanol:water, and chloroform). Samples were then centrifuged at 14 300 rpm at 4 °C for 25 min. The aqueous layer was transferred into a new microfuge tube, concentrated in the Speed-Vac SPD120 (Thermo Fisher Scientific) and dried under nitrogen stream, followed by reconstitution in the acetonitrile/methanol/formic acid (75:25:0.2; v/v/v, HPLC grade; Thermo Fisher Scientific) for the HILIC analysis, and acetonitrile/methanol (25:25; v/v/v, HPLC grade) for the AMIDE analysis. |