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MB Sample ID: SA339256
Local Sample ID: | ColGF7.2 |
Subject ID: | SU003249 |
Subject Type: | Mammal |
Subject Species: | Mus musculus |
Taxonomy ID: | 10090 |
Genotype Strain: | C57Bl/6J |
Age Or Age Range: | 8-10 weeks |
Weight Or Weight Range: | 20-29g |
Gender: | Male |
Animal Animal Supplier: | Taconic |
Animal Housing: | Gnotobiotic isolators/conventional housing |
Animal Light Cycle: | 12/12 |
Animal Feed: | RM1A (P) and RM3A (P) from Special Diet Services |
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Sample Preparation:
Sampleprep ID: | SP003256 |
Sampleprep Summary: | Samples were prepared using the automated MicroLab STARĀ® system from Hamilton Company. Several recovery standards were added prior to the first step in the extraction process for QC purposes. To remove protein, dissociate small molecules bound to protein or trapped in the precipitated protein matrix, and to recover chemically diverse metabolites, proteins were precipitated with methanol under vigorous shaking for 2 min (Glen Mills GenoGrinder 2000) followed by centrifugation. The resulting extract was divided into five fractions: two for analysis by two separate reverse phase (RP)/UPLC-MS/MS methods with positive ion mode electrospray ionization (ESI), one for analysis by RP/UPLC-MS/MS with negative ion mode ESI, one for analysis by HILIC/UPLC-MS/MS with negative ion mode ESI, and one sample was reserved for backup. Samples were placed briefly on a TurboVapĀ® (Zymark) to remove the organic solvent. The sample extracts were stored overnight under nitrogen before preparation for analysis. |