Metabolomics Workbench : NIH Data Repository

MB Sample ID: SA340631

Local Sample ID:	VM54_Adjacent_Kidney
Subject ID:	SU003260
Subject Type:	Human
Subject Species:	Homo sapiens
Taxonomy ID:	9606
Gender:	Male and female

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Subject
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Collection
Treatment
Sample Preparation
Chromatography
Analysis
MS

Sample Preparation:

Sampleprep ID:	SP003267
Sampleprep Summary:	Frozen tissue fragments weighing 10-30mg were added to ice cold 80:20 methanol:water and extracted for metabolomics analysis. Samples were subjected to three freeze-thaw cycles, then centrifuged at 16,000xg for 20 minutes to precipitate macromolecules. The supernatant was evaporated using a vacuum concentrator. Samples were resuspended in 100 μL of 0.1% formic acid in water, vortexed for 30 seconds, and centrifuged at 16,000g for 15 minutes. Supernatant was transferred to an autosampler vial and then run on the MS.

Sampleprep ID:

SP003267

Sampleprep Summary:

Frozen tissue fragments weighing 10-30mg were added to ice cold 80:20 methanol:water and extracted for metabolomics analysis. Samples were subjected to three freeze-thaw cycles, then centrifuged at 16,000xg for 20 minutes to precipitate macromolecules. The supernatant was evaporated using a vacuum concentrator. Samples were resuspended in 100 μL of 0.1% formic acid in water, vortexed for 30 seconds, and centrifuged at 16,000g for 15 minutes. Supernatant was transferred to an autosampler vial and then run on the MS.