Metabolomics Workbench : NIH Data Repository

MB Sample ID: SA341104

Local Sample ID:	CRC-tissue-C0.43.fid#
Subject ID:	SU003272
Subject Type:	Human
Subject Species:	Homo sapiens
Taxonomy ID:	9606

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Sample Preparation:

Sampleprep ID:	SP003279
Sampleprep Summary:	Frozen tissue samples (approximately 300 mg) were thawed at 25°C, then sectioned and homogenized at 16,000 rpm for 80 seconds in a solution consisting of 0.6 mL distilled water and 1.2 mL methanol. Subsequently, chloroform (1.2 mL) and distilled water (1.2 mL) were added and the sample was vortexed for 60 seconds. Finally, samples were incubated on ice for 15 mins, followed by centrifugation at 2000 rpm for 5 mins. The resulting supernatant was washed with nitrogen and evaporated before incubating under vacuum for at least 18 hours. The subsequent lyophilized powder was dissolved in 550 μL PBS/D2O buffer (0.1 M, pH 7.4) and 50 μL TSP/D2O stock solution, and after centrifugation at 10,000 rpm for 5 mins,500 μL of the supernatant was transferred to an NMR tube (5 mm size) for 1H NMR spectroscopy analysis.

Sampleprep ID:

SP003279

Sampleprep Summary:

Frozen tissue samples (approximately 300 mg) were thawed at 25°C, then sectioned and homogenized at 16,000 rpm for 80 seconds in a solution consisting of 0.6 mL distilled water and 1.2 mL methanol. Subsequently, chloroform (1.2 mL) and distilled water (1.2 mL) were added and the sample was vortexed for 60 seconds. Finally, samples were incubated on ice for 15 mins, followed by centrifugation at 2000 rpm for 5 mins. The resulting supernatant was washed with nitrogen and evaporated before incubating under vacuum for at least 18 hours. The subsequent lyophilized powder was dissolved in 550 μL PBS/D2O buffer (0.1 M, pH 7.4) and 50 μL TSP/D2O stock solution, and after centrifugation at 10,000 rpm for 5 mins,500 μL of the supernatant was transferred to an NMR tube (5 mm size) for 1H NMR spectroscopy analysis.