Metabolomics Workbench : NIH Data Repository

MB Sample ID: SA343020

Local Sample ID:	4
Subject ID:	SU003284
Subject Type:	Mammal
Subject Species:	Rattus norvegicus
Taxonomy ID:	10116
Gender:	Male

Select appropriate tab below to view additional metadata details:

All
Subject
Factors
Collection
Treatment
Sample Preparation
Chromatography
Analysis
MS

Sample Preparation:

Sampleprep ID:	SP003291
Sampleprep Summary:	Sagittal 12-µm sections collected serially from right brain hemispheres using a cryostat (Thermo Shandon NX70 Cryostar, Waltham, MA) were mounted onto indium-tin-oxide (ITO) slides (Delta Technologies, Loveland, CO) and stored at -80°C until MALDI MSI. Prior to imaging, slides were placed in a desiccator for 15 minutes and sprayed with 8 passes of a 5 mg mL-1 DAN solution in 90% acetonitrile/10% water using an HTX TM-Sprayer (HTX Technologies, Chapel Hill, NC) at 30°C, 0.1 mL min-1 flow rate, 1200 mm min-1 velocity, 2.5 mm tracking speed, 10 psi, 2 L min-1 gas flow rate, 0 second drying time and 40 mm nozzle height.

Sampleprep ID:

SP003291

Sampleprep Summary:

Sagittal 12-µm sections collected serially from right brain hemispheres using a cryostat (Thermo Shandon NX70 Cryostar, Waltham, MA) were mounted onto indium-tin-oxide (ITO) slides (Delta Technologies, Loveland, CO) and stored at -80°C until MALDI MSI. Prior to imaging, slides were placed in a desiccator for 15 minutes and sprayed with 8 passes of a 5 mg mL-1 DAN solution in 90% acetonitrile/10% water using an HTX TM-Sprayer (HTX Technologies, Chapel Hill, NC) at 30°C, 0.1 mL min-1 flow rate, 1200 mm min-1 velocity, 2.5 mm tracking speed, 10 psi, 2 L min-1 gas flow rate, 0 second drying time and 40 mm nozzle height.