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MB Sample ID: SA343103
| Local Sample ID: | 21796944 |
| Subject ID: | SU003290 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Age Or Age Range: | >14 days, |
| Gender: | Male and female |
| Human Inclusion Criteria: | >14 days, MSUD sick patients DBS |
| Human Exclusion Criteria: | </=14 days, any IEM's sick other than MSUD, unknown gender |
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Sample Preparation:
| Sampleprep ID: | SP003297 |
| Sampleprep Summary: | One punch, of size 3.3mm, DBS from MSUD newborn and healthy controls were distributed in 96 V-shaped plate wells, then immersed with 250 μL of extraction solvent composed of 20% Water: 20% MeOH:40% ACN. The samples were vortexed in thermomixer (Eppendrof, Germany) at 600 rpm, 25˚C, for 2hrs. The samples were spun down at 16.000 rpm, 4˚C, for 10 min. The supernatants were transferred into new 96 V-shaped plate and the punches discarded, and then the samples were dried in a vacuum concentrator SpeedVac (Christ, City, Germany). Dry residue was re-dissolved in 100 μL of methanol/water with a ratio (1:1) prior to LC-MS analysis. |
| Sampleprep Protocol Filename: | MSUD_Metabolites_Extraction.pdf |
| Processing Storage Conditions: | Room temperature |
| Extract Storage: | Room temperature |
