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MB Sample ID: SA343153
Local Sample ID: | Enzyme assay_Apple pear hybrid_HybASR2 |
Subject ID: | SU003292 |
Subject Type: | Plant |
Subject Species: | Malus domestica/Pyrus communis/Apple-pear intergeneric hybrid |
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Sample Preparation:
Sampleprep ID: | SP003299 |
Sampleprep Summary: | For phenolic targeted profiling, 100 mg of fresh tissue (FW) was extracted in 4 mL 80% v·v-1 methanol, sonicated for 20 min at 60 Hz in a water bath at 25ºC, agitated for further 20 min and kept in dark for 48 h, filtered through a 0.22 µm PTFE filter and stored at 4 ºC. For recombinant protein assays, each 200 µL reaction was extracted with 300 µL methanol for injection. |