Metabolomics Workbench : NIH Data Repository

MB Sample ID: SA344200

Local Sample ID:	NEG_575
Subject ID:	SU003296
Subject Type:	Human
Subject Species:	Homo sapiens
Taxonomy ID:	9606

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Sample Preparation:

Sampleprep ID:	SP003303
Sampleprep Summary:	The plasma samples were thawed by transferring them from -80°C to a 4°C refrigerator. Then, take out 50 μL of the plasma sample into another tube after vortex mixing and added to 250 μL of pre-cooled Spike MeOH containing isotopic chemicals (120.89 μM 13C6-D-glucose, 23.12 mM 13C5-L-glutamate-15N). The mixture was thoroughly mixed by vortexing at 1500 rpm for 2 minutes at 4°C. The mixture was then placed in a -20°C refrigerator and allowed to stand for 30 minutes. Afterward, ultrasonication was performed in an ice-water bath for 10 minutes. Following ultrasonication, the mixture was centrifuged at 13,000 rpm for 20 minutes at 4°C. 20 μL of each sample from every batch was extracted from the tube and mixed for QC analysis using mass spectrometry (MS). The 150ul remaining extract was concentrated, vacuum dried, and stored at -80°C.

Sampleprep ID:

SP003303

Sampleprep Summary:

The plasma samples were thawed by transferring them from -80°C to a 4°C refrigerator. Then, take out 50 μL of the plasma sample into another tube after vortex mixing and added to 250 μL of pre-cooled Spike MeOH containing isotopic chemicals (120.89 μM 13C6-D-glucose, 23.12 mM 13C5-L-glutamate-15N). The mixture was thoroughly mixed by vortexing at 1500 rpm for 2 minutes at 4°C. The mixture was then placed in a -20°C refrigerator and allowed to stand for 30 minutes. Afterward, ultrasonication was performed in an ice-water bath for 10 minutes. Following ultrasonication, the mixture was centrifuged at 13,000 rpm for 20 minutes at 4°C. 20 μL of each sample from every batch was extracted from the tube and mixed for QC analysis using mass spectrometry (MS). The 150ul remaining extract was concentrated, vacuum dried, and stored at -80°C.