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MB Sample ID: SA345851
Local Sample ID: | KAE609_20x_3 |
Subject ID: | SU003298 |
Subject Type: | Cultured cells |
Subject Species: | Plasmodium falciparum |
Taxonomy ID: | 5833 |
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Sample Preparation:
Sampleprep ID: | SP003305 |
Sampleprep Summary: | Following compound incubation, cultures were centrifuged at 1,200 g for 3 min, the media was removed, and the cell pellets were washed in 1 mL of ice-cold PBS. Samples were again centrifuged at 1,200 g for 3 min to remove all of the PBS and metabolites were extracted from 5 x 107 cells using 90 µL of ice-cold methanol extraction solvent. Samples were then incubated on an automatic vortex mixer at 4 °C for 1 h before being centrifuged at 21,000 g for 10 min. The supernatants were transferred into high-performance liquid chromatography (HPLC) vials and stored at -80 °C until liquid chromatography-mass spectrometry (LC-MS) analysis. A 10 µL aliquot from each sample was pooled to serve as a quality control sample for monitoring instrument reproducibility and to aid downstream metabolite identification. |