Metabolomics Workbench : NIH Data Repository

MB Sample ID: SA345852

Local Sample ID:	KAE609_20x_2
Subject ID:	SU003298
Subject Type:	Cultured cells
Subject Species:	Plasmodium falciparum
Taxonomy ID:	5833

Select appropriate tab below to view additional metadata details:

Sample Preparation:

Sampleprep ID:	SP003305
Sampleprep Summary:	Following compound incubation, cultures were centrifuged at 1,200 g for 3 min, the media was removed, and the cell pellets were washed in 1 mL of ice-cold PBS. Samples were again centrifuged at 1,200 g for 3 min to remove all of the PBS and metabolites were extracted from 5 x 107 cells using 90 µL of ice-cold methanol extraction solvent. Samples were then incubated on an automatic vortex mixer at 4 °C for 1 h before being centrifuged at 21,000 g for 10 min. The supernatants were transferred into high-performance liquid chromatography (HPLC) vials and stored at -80 °C until liquid chromatography-mass spectrometry (LC-MS) analysis. A 10 µL aliquot from each sample was pooled to serve as a quality control sample for monitoring instrument reproducibility and to aid downstream metabolite identification.

Sampleprep ID:

SP003305

Sampleprep Summary:

Following compound incubation, cultures were centrifuged at 1,200 g for 3 min, the media was removed, and the cell pellets were washed in 1 mL of ice-cold PBS. Samples were again centrifuged at 1,200 g for 3 min to remove all of the PBS and metabolites were extracted from 5 x 107 cells using 90 µL of ice-cold methanol extraction solvent. Samples were then incubated on an automatic vortex mixer at 4 °C for 1 h before being centrifuged at 21,000 g for 10 min. The supernatants were transferred into high-performance liquid chromatography (HPLC) vials and stored at -80 °C until liquid chromatography-mass spectrometry (LC-MS) analysis. A 10 µL aliquot from each sample was pooled to serve as a quality control sample for monitoring instrument reproducibility and to aid downstream metabolite identification.