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MB Sample ID: SA346684
Local Sample ID: | 20210111_LC_33 |
Subject ID: | SU003300 |
Subject Type: | Human |
Subject Species: | Homo sapiens |
Taxonomy ID: | 9606 |
Gender: | Female |
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Sample Preparation:
Sampleprep ID: | SP003307 |
Sampleprep Summary: | Aliquots of 50 µL of plasma were added into a 96-deep well plate cell to which 1 µL of thiol-derivatization solution (200 mM N-ethylmaleimide (NEM) + 2 mM citric acid) was added. Subsequently, 200 µL of precipitation solution (acetonitrile/isopropanol (7:3, v/v) + 1% formic acid + isotopically labelled internal standards) were added. After vortex agitation for 10 min. and refrigeration at -20ºC for 10 minutes, the samples were centrifuged (14.000 rpm for 10 minutes at 4°C). |