Metabolomics Workbench : NIH Data Repository

MB Sample ID: SA346721

Local Sample ID:	Sen_shHK2_WTHK2_1
Subject ID:	SU003301
Subject Type:	Cultured cells
Subject Species:	Homo sapiens
Taxonomy ID:	9606

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Sample Preparation:

Sampleprep ID:	SP003308
Sampleprep Summary:	Following incubation, the cells were washed with chilled PBS and incubated with 500 µl of extraction solution (80:20 v/v methanol/water) at 4 °C for 5 minutes. Next, cells were scraped with a polypropylene cell scraper and the extraction solution from each sample was collected, vortexed, and incubated on dry ice for at least 30 min. Then, each sample was centrifuged at maximum speed at 4 °C for 10 minutes, and the resulting supernatant was used for metabolic measurements.

Sampleprep ID:

SP003308

Sampleprep Summary:

Following incubation, the cells were washed with chilled PBS and incubated with 500 µl of extraction solution (80:20 v/v methanol/water) at 4 °C for 5 minutes. Next, cells were scraped with a polypropylene cell scraper and the extraction solution from each sample was collected, vortexed, and incubated on dry ice for at least 30 min. Then, each sample was centrifuged at maximum speed at 4 °C for 10 minutes, and the resulting supernatant was used for metabolic measurements.