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MB Sample ID: SA346773
Local Sample ID: | DS2-039-009 |
Subject ID: | SU003303 |
Subject Type: | Mammal |
Subject Species: | Mus musculus |
Gender: | Not applicable |
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Sample Preparation:
Sampleprep ID: | SP003310 |
Sampleprep Summary: | For tumor analysis, tissue was weighed and placed into 1.5mL Eppendorf Safe-Lock tubes that were pre-filled with 1.0mm glass grinder beads (Biospec Products Inc). Cold methanol was then added to each tube so that all final tissue concentrations were 20 mg/mL. Samples were then homogenized via a “Next Advance” Bullet Blender for 5 minutes and subsequently vortexed for 30 minutes. Following vortex, insoluble material was pelleted by centrifugation (18000g, 10 min) and supernatants were isolated for analysis by UHPLC-MS. |
Processing Storage Conditions: | 4℃ |
Extract Storage: | -80℃ |