Metabolomics Workbench : NIH Data Repository

MB Sample ID: SA352106

Local Sample ID:	cMIFKO-IR_2
Subject ID:	SU003341
Subject Type:	Mammal
Subject Species:	Mus musculus
Taxonomy ID:	10090

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Sample Preparation:

Sampleprep ID:	SP003348
Sampleprep Summary:	The lipidomic sample preparation method was: The samples were thawed on ice. Approximately 10 mg of each sample was weighed and homogenized by ball-mill in 1mL of extraction solution (MTBE:Methanol = 3:1, V/V) with internal standards. The mixture was vortexed for 15 min. Next, the mixture was added with 200 µL of water and vortexed for 1 min and incubating at 4 °C for 10 min. After centrifugation at 12000 rpm for 10 min (4 °C), 200 µL of the upper phase was collected for complete solvent drying under 20 °C. The residue was reconstituted using 200 µL of reconstitution solution (ACN:IPA = 1:1, V/V), followed by vortex for 3 min and centrifugation at 12000 rpm for 3 min. A 120 µL of the final supernatant was used for LC-MS analysis. The data acquisition instruments consisted of Ultra Performance Liquid Chromatography (UPLC) (Nex-era LC-40) and tandem mass spectrometry (MS/MS) (Triple Quad 6500+).

Sampleprep ID:

SP003348

Sampleprep Summary:

The lipidomic sample preparation method was: The samples were thawed on ice. Approximately 10 mg of each sample was weighed and homogenized by ball-mill in 1mL of extraction solution (MTBE:Methanol = 3:1, V/V) with internal standards. The mixture was vortexed for 15 min. Next, the mixture was added with 200 µL of water and vortexed for 1 min and incubating at 4 °C for 10 min. After centrifugation at 12000 rpm for 10 min (4 °C), 200 µL of the upper phase was collected for complete solvent drying under 20 °C. The residue was reconstituted using 200 µL of reconstitution solution (ACN:IPA = 1:1, V/V), followed by vortex for 3 min and centrifugation at 12000 rpm for 3 min. A 120 µL of the final supernatant was used for LC-MS analysis. The data acquisition instruments consisted of Ultra Performance Liquid Chromatography (UPLC) (Nex-era LC-40) and tandem mass spectrometry (MS/MS) (Triple Quad 6500+).