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MB Sample ID: SA353363
Local Sample ID: | 26-08-18-2022-POS-Isaac-S03 |
Subject ID: | SU003362 |
Subject Type: | Mammal |
Subject Species: | Mus musculus |
Taxonomy ID: | 10090 |
Gender: | Male and female |
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Sample Preparation:
Sampleprep ID: | SP003369 |
Sampleprep Summary: | For serum for WT, liver-specific Gclc KO, liver-specific Nrf2 KO, and liver-specific Gclc-Nrf2 DKO, 20 µL of mouse serum was combined with 180 µL of chloroform:methanol extraction solvent (v:v=1:2) containing internal standards at the final concentrations: 5nM D7-Sphinganine (Avanti Polar Lipids Inc., Cat# 860658), 12.5nM D3-Deoxysphinganine (Avanti Polar Lipids Inc., Cat# 860474), and SPLASH LIPIDOMIX (1:1000, Avanti Polar Lipids Inc., Cat# 330707). After sonicating (1400 rpm, 20°C, 5 min), the extracts were cleared by centrifugation (17,000g, 20°C, 10 min), and the lipids in the supernatant were analyzed by LC-MS. |