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MB Sample ID: SA353378
Local Sample ID: | MI 3d-9 |
Subject ID: | SU003363 |
Subject Type: | Mammal |
Subject Species: | Mus musculus |
Taxonomy ID: | 10090 |
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Sample Preparation:
Sampleprep ID: | SP003370 |
Sampleprep Summary: | 50 mg of spleen tissues was homogenized with methanol (2% formic acid and 0.01 mol/L BHT) spiked with internal standard mixture. After centrifugation, the supernatant was added to water and ethylacetate, and the sample was mixed and centrifuged at 12,000 × g. The upper organic phase was transferred to a new tube, and the water phase was extracted again. The organic phase was combined and then evaporated to dryness and further dissolved in 100 μL of 30% acetonitrile. After vigorous mixing, samples were filtered by use of centrifuge tube filters (nylon membrane, 0.22 μm). |