Metabolomics Workbench : NIH Data Repository

MB Sample ID: SA353379

Local Sample ID:	MI 3d-8
Subject ID:	SU003363
Subject Type:	Mammal
Subject Species:	Mus musculus
Taxonomy ID:	10090

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Sample Preparation
Chromatography
Analysis
MS

Sample Preparation:

Sampleprep ID:	SP003370
Sampleprep Summary:	50 mg of spleen tissues was homogenized with methanol (2% formic acid and 0.01 mol/L BHT) spiked with internal standard mixture. After centrifugation, the supernatant was added to water and ethylacetate, and the sample was mixed and centrifuged at 12,000 × g. The upper organic phase was transferred to a new tube, and the water phase was extracted again. The organic phase was combined and then evaporated to dryness and further dissolved in 100 μL of 30% acetonitrile. After vigorous mixing, samples were filtered by use of centrifuge tube filters (nylon membrane, 0.22 μm).

Sampleprep ID:

SP003370

Sampleprep Summary:

50 mg of spleen tissues was homogenized with methanol (2% formic acid and 0.01 mol/L BHT) spiked with internal standard mixture. After centrifugation, the supernatant was added to water and ethylacetate, and the sample was mixed and centrifuged at 12,000 × g. The upper organic phase was transferred to a new tube, and the water phase was extracted again. The organic phase was combined and then evaporated to dryness and further dissolved in 100 μL of 30% acetonitrile. After vigorous mixing, samples were filtered by use of centrifuge tube filters (nylon membrane, 0.22 μm).