Metabolomics Workbench : NIH Data Repository

MB Sample ID: SA353543

Local Sample ID:	S3_37_2h
Subject ID:	SU003370
Subject Type:	Bacteria
Subject Species:	Mycoplasma mycoides, Minimal cell JCVI-syn3B

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Sample Preparation:

Sampleprep ID:	SP003377
Sampleprep Summary:	Bacterial samples are harvested at designated time points from the continuous cell culture in the bioreactor system and washed twice at their growth temperature in mycoplasma wash buffer (200 mM NaCl, 25 mM HEPES, 1% glucose, pH 7.0) to remove traces of the growth medium (21000g, 2 min centrifuging steps). Washed cell pellet is resuspended in 150 uL PBS and flash-frozen in liquid nitrogen. Lipids are extracted using a two-step chloroform/methanol procedure (Bligh-Dyer lipid extraction protocol)

Sampleprep ID:

SP003377

Sampleprep Summary:

Bacterial samples are harvested at designated time points from the continuous cell culture in the bioreactor system and washed twice at their growth temperature in mycoplasma wash buffer (200 mM NaCl, 25 mM HEPES, 1% glucose, pH 7.0) to remove traces of the growth medium (21000g, 2 min centrifuging steps). Washed cell pellet is resuspended in 150 uL PBS and flash-frozen in liquid nitrogen. Lipids are extracted using a two-step chloroform/methanol procedure (Bligh-Dyer lipid extraction protocol)