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MB Sample ID: SA354269
| Local Sample ID: | K3 |
| Subject ID: | SU003385 |
| Subject Type: | Cultured cells |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
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Sample Preparation:
| Sampleprep ID: | SP003392 |
| Sampleprep Summary: | For metabolite analysis, naive ME2+/+ and ME2-/- CD8+ T cells were isolated and activated with anti-CD3 (4μg/ml) and anti-CD28 (1μg/ml). Cells were washed twice with ice-cold PBS and metabolites were extracted with ice-cold 80% methanol. The extracts were analyzed by LC-MS/MS. |
| Extraction Method: | Cells were washed twice with ice-cold PBS and metabolites were extracted with ice-cold 80% methanol. |
| Extract Storage: | -80℃ |
