Summary of Study ST000055
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000052. The data can be accessed directly via it's Project DOI: 10.21228/M8H01X This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
Study ID | ST000055 |
Study Title | Effect of kinase inhibitors on FLT3-ITD AML cell metabolomes |
Study Summary | FLT3-ITD AML cells (MR2) obtained from mice were treated with two MEK kinase inhibitors (GSK and AZD) at 10 uM versus media only control. Conditioned media aliquots and cellular fractions comprised of two aliquots (tecnical replicates) for LC-MS metabolomic and one for Western blot analyses were collected at 0, 4, 24, and 48 hours. The experiment was repeated three times. HPLC-MS data were acquired for 24 samples from one biological experiment. |
Institute | University of North Carolina |
Department | Systems and Translational Sciences |
Laboratory | Sumner Lab |
Last Name | Sumner |
First Name | Susan |
Address | Eastern Regional Comprehensive Metabolomics Resource Core, UNC Nutrition Research Institute, 500 Laureate Way, Kannapolis, NC, 28081 |
susan_sumner @unc.edu | |
Phone | 704-250-5066 |
Submit Date | 2014-06-06 |
Num Groups | 12 |
Total Subjects | 24 |
Raw Data Available | Yes |
Raw Data File Type(s) | raw(Waters) |
Uploaded File Size | 1.2 G |
Analysis Type Detail | LC-MS |
Release Date | 2015-06-01 |
Release Version | 1 |
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Sample Preparation:
Sampleprep ID: | SP000070 |
Sampleprep Summary: | Samples were extracted using 90:10 methanol:chloroform. For reversed phase, samples were dried down and reconstituted in 95:5 water:methanol. For HILIC, samples were directly transferred to autosampler vials. |
Sampleprep Protocol Filename: | RTI_GRAVES_Metabolomics_Procedure.docx |
Extraction Method: | 90:10 methanol:chloroform |
Extract Storage: | -80 C |
Sample Resuspension: | 95:5 Water:Methanol |
Sample Spiking: | L-Tryptophan-d5 |
Cell Type: | FLT3-ITD AML (myeloid) |