Summary of Study ST000133
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000118. The data can be accessed directly via it's Project DOI: 10.21228/M8B594 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
Study ID | ST000133 |
Study Title | 1H NMR Metabolomics Study of Metastatic Melanoma in C57BL/6J Mouse Spleen |
Study Type | Tissue Extracts Comparison |
Study Summary | Tissue extracts from metastatic melanoma mouse spleen and controls were compared via NMR based metabolomic analysis |
Institute | Pacific Northwest National Laboratory |
Department | Fundamental & Computational Sciences |
Last Name | Hu |
First Name | Jianzhi |
jianzhi.hu@pnnl.gov | |
Submit Date | 2015-01-08 |
Num Groups | 1 |
Total Subjects | 12 |
Raw Data Available | Yes |
Uploaded File Size | 140 K |
Analysis Type Detail | NMR |
Release Date | 2015-01-08 |
Release Version | 1 |
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Sample Preparation:
Sampleprep ID: | SP000149 |
Sampleprep Summary: | Cold Methanol/H2O/Chloroform Extraction |
Extraction Method: | Step 1: The pre-weighted intact frozen tissue was homogenized in ice bath after adding 0.25 ml MeOH and 0.053 ml H2O for each sample containing 30 to 60 mg of tissue, followed by vortexing the mixture and then adding 0.125 ml chloroform, vortexing again. Step 2: 0.125 ml chloroform and 0.125 ml water was added to the sample and then vortexed again, followed by transferring different layers into glass vials separately with syringes after the mixture being left on ice for 15 min and centrifuged at 8000rpm for 2 min at 4°C. Finally, the solvents were removed by employing lyophilizer (MeOH/H2O layer, hydrophilic metabolites) or by evaporating under dry nitrogen gas (CHCl3 layer, hydrophobic metabolites). The extracts were then stored at -80°C before performing NMR measurements. |