Summary of Study ST000137
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000122. The data can be accessed directly via it's Project DOI: 10.21228/M8T59G This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
Study ID | ST000137 |
Study Title | Metabolomics in sarcoidosis |
Study Type | observation |
Study Summary | Metabolites in sarcoidosis patients |
Institute | Wayne State University |
Department | Internal Medicine |
Last Name | Geamanu |
First Name | Andreea |
ageamanu@med.wayne.edu | |
Submit Date | 2015-02-11 |
Total Subjects | 30 |
Raw Data Available | No |
Analysis Type Detail | NMR |
Release Date | 2015-02-11 |
Release Version | 1 |
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Sample Preparation:
Sampleprep ID: | SP000153 |
Sampleprep Summary: | Serum samples were analyzed following filtration through a 3-kD nominal cut-off membrane (Amicon Ultra 0.5 mL centrifugal filters, Millipore Corp, Billerica, MA) to remove proteins, lipids, lipoproteins, and protein-bound forms of certain molecules. Each serum sample was transferred to a prewashed filter and centrifuged at 13,800 g for 60 minutes as previously described (Gregory, Park et al. 2013). The filters were further washed with deuterium oxide (D2O) and the filtrate was mixed with the filtered serum in a 1:1 ratio. A reference buffer solution containing 5 mmol/L disodium-2,2-dimethyl 2-silapentane-5-sulphonate (DSS) and 10 mmol/L imidazole in D2O was added to the samples in a 1:9 ratio. The D2O provided a field-frequency lock, whereas DSS was used as the chemical shift reference and imidazole as pH indicator for the NMR spectra. A total of 600 µL mixture was transferred to a 5 mm NMR tube (Sigma Aldrich, St. Louis, MO). |