Summary of Study ST000221
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000181. The data can be accessed directly via it's Project DOI: 10.21228/M8R30R This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
Study ID | ST000221 |
Study Title | Normal plasma cells,Low proliferation multiple myeloma and High proliferation multiple myeloma cells |
Study Type | differential metabolomics |
Study Summary | CD138 sorted bone marrow plasma cell were obtained from a normal patient, a multiple myelow with slow proliferation and a multiple myelow with rapid proliferation. |
Institute | Mayo Clinic |
Department | Hematology |
Last Name | Gonsalves |
First Name | William |
Dasari.Surendra@mayo.edu | |
Submit Date | 2015-06-20 |
Num Groups | 1 |
Total Subjects | 1 |
Raw Data Available | Yes |
Raw Data File Type(s) | d |
Uploaded File Size | 73 G |
Analysis Type Detail | LC-MS |
Release Date | 2016-06-18 |
Release Version | 1 |
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Sample Preparation:
Sampleprep ID: | SP000242 |
Sampleprep Summary: | 1) Incubated cell pellets on ice for 15 min 2) Added PBS to cells and vortex well 3) Sonicate for 1 min in ice bath 4) Added MeOH+ACN mixture to cells and vortex well 5) Sonicated for 1 min in ice bath 6) Incubated on ice for 30 min + spin for 20 min at 18K RPM 7) Combined 110 ul from each sample into a pool sample (330 ul total) 8) Split pool into two fractions of 150 ul each (hilic & C18) 9) Split remaning sample into 150 ul x 2 (hilic & C18) |