Summary of Study ST000450
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000348. The data can be accessed directly via it's Project DOI: 10.21228/M82K58 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
Study ID | ST000450 |
Study Title | Metabolic features of chronic fatigue syndrome |
Study Type | Plasma metabolomic profiling |
Study Summary | This targeted metabolomic analysis was performed on plasma samples from 39 normal controls (n=18 men and 21 women) and 45 subjects ((n = 22 men and 23 women) who met diagnostic criteria for ME/CFS by Institute of Medicine, Canadian, and Fukuda criteria. |
Institute | University of California, San Diego |
Department | The Mitochondrial and Metabolic Disease Center |
Last Name | Naviaux |
First Name | Robert |
Address | 214 Dikinson Street, CTF-C102, San Diego, CA, 92103 |
maviaux@ucsd.edu | |
Phone | 619-993-2904 |
Submit Date | 2016-08-11 |
Num Groups | 2 groups for men (control and CFS) and 2 groups for women (control and CFS) |
Total Subjects | 84 |
Raw Data File Type(s) | wiff |
Analysis Type Detail | LC-MS |
Release Date | 2016-12-22 |
Release Version | 1 |
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Sample Preparation:
Sampleprep ID: | SP000478 |
Sampleprep Summary: | None |
Extraction Method: | Typically, 90 μl of plasma was thawed on ice and mixed with 5 μl of isotope standards and incubated for 10 min at 20°C to permit small molecules and vitamins in the internal standards to associate with plasma binding proteins. Macromolecules (protein, DNA, RNA, glycans, etc.) were precipitated by extraction with 4 volumes (400 μl) of cold (-20°C), acetonitrile:methanol (50:50) and incubated on crushed ice for 10 min, then removed by centrifugation at 16,000g x 10 min at 4°C. The supernatants containing the extracted metabolites and internal standards were transferred to labeled cryotubes and stored at -80°C for LCMS/MS analysis. |
Extract Storage: | -80°C |
Sample Derivatization: | No |
Sample Spiking: | 25-35 commercial stable isotope internal standards, and 57 stable isotope internal standards that were custom-synthesized in E. coli and S. cerevisiae by metabolic labeling with 13C-glucose and 13C bicarbonate |