Summary of Study ST000569
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000417. The data can be accessed directly via it's Project DOI: 10.21228/M8531V This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
Study ID | ST000569 |
Study Title | Effect of minimal and complex media on the metabolite profiles |
Study Summary | Media dependent intracellular metabolite changes of microorganisms |
Institute | Graduate school of Korea University |
Last Name | Kim |
First Name | Jungyeon |
Address | 145, Anam-ro, Seongbuk-gu, Seoul, Seoul, 02841, Korea, South |
kim131812@korea.ac.kr | |
Phone | 821082248015 |
Submit Date | 2017-03-07 |
Study Comments | Each class means,Intracellular metabolites of E.coli cultivated in M9 Intracellular metabolites of E.coli cultivated in LB Intracellular metabolites of S.cerevisiae cultivated in YNB Intracellular metabolites of S.cerevisiae cultivated in YPD. |
Raw Data Available | Yes |
Raw Data File Type(s) | peg |
Analysis Type Detail | GC-MS |
Release Date | 2017-07-10 |
Release Version | 1 |
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Sample Preparation:
Sampleprep ID: | SP000598 |
Sampleprep Summary: | metabolite samples were derivatized in two steps. First, the samples were incubated with 5 µl of 40 mg/ml methoxyamine hydrochloride in pyridine (Sigma-Aldrich, St. Louis, MO) at 30 °C for 90 min, and then, with 45 µl of N-methyl-N-trimethylsilyl-trifluoroacetamide (Fluka, Buchs, Switzerland) at 37 °C for 30 min. A mixture of fatty acid methyl esters including methyl forms of C8, C9, C10, C12, C14, C16, C18, C20, C22, C24, C26, C28, and C30 was added to the derivatized sample as the retention index markers. |