Summary of Study ST000903
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000627. The data can be accessed directly via it's Project DOI: 10.21228/M8MT2H This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
Study ID | ST000903 |
Study Title | Pharmacometabolomic analysis of rat hippocampus and plasma in response to chronic stress and albiflorin treament |
Study Type | Animal study |
Study Summary | Male Sprague-Dawley (SD) rats were exposed to one random stressor per day for 35 d. Following four weeks of stress exposure, rats with stress-induced depression-like behaviors were treated daily with either saline, fluoxetine or albiflorin for 7 days via intragastric gavage. Rat heparinized plasma was collected after drug treatment. After behavior tests, rats were sacrificed and the hippocampus was collected. Pharmacometabolomic analysis was conducted for both plasma and hippocampal samples |
Institute | Beijing Wonner Biotech. Ltd. Co. |
Last Name | Zhang |
First Name | Zuoguang |
Address | No.9, Kexing Road, Fengtai, Beijing, Beijing, 101111, China |
leekefeng@hotmail.com | |
Phone | 86-10-65670787 |
Submit Date | 2017-11-16 |
Num Groups | 4 |
Study Comments | Animal study |
Analysis Type Detail | LC-MS |
Release Date | 2018-02-07 |
Release Version | 1 |
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Sample Preparation:
Sampleprep ID: | SP000947 |
Sampleprep Summary: | Briefly, for plasma samples, 95 µl of plasma was mixed with 5 µl of isotope standards mix and extracted with 400 µl of pre-cold extraction buffer containing methanol/acetonitrile (50/50, v/v). The mixture was incubated on ice for 30 min and centrifuged for 10 min at 16,000 g and 4 ºC. The supernatant containing both polar and nonpolar metabolites was transferred to new tubes and stored at -80 ºC for metabolomic analysis |
Extraction Method: | methanol/acetonitrile (50/50, v/v) precipitation |
Extract Storage: | -80 ºC |
Sample Derivatization: | No |
Sample Spiking: | Isotope standards |