Summary of Study ST001019
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000682. The data can be accessed directly via it's Project DOI: 10.21228/M8HQ2J This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
Study ID | ST001019 |
Study Title | Lipidomic profiling of heart and plasma of mice following swim training versus pressure overload |
Study Summary | Lipid profiling was performed on hearts and plasma from mice subjected to a physiological stimulus (4 weeks of swim exercise training) or pathological stimulus (4 weeks of pressure overload – transverse aortic constriction; TAC) |
Institute | Baker Heart and Diabetes Institute |
Laboratory | Cardiac Hypertrophy |
Last Name | Tham |
First Name | Yow Keat |
Address | 75 Commercial Rd, Melbourne 3004 |
yowkeat.tham@baker.edu.au | |
Phone | +65385321266 |
Submit Date | 2018-07-15 |
Raw Data File Type(s) | d |
Analysis Type Detail | LC-MS |
Release Date | 2018-07-25 |
Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Sample Preparation:
Sampleprep ID: | SP001065 |
Sampleprep Summary: | Cardiac tissue samples were homogenized and sonicated in phosphate buffered saline (PBS) (pH 7.4). The Bicinchoninic acid (BCA) assay (Thermo Scientific) was used to determine protein concentration. A mixture of internal standards (10µl) in CHCl3: methanol (1:1) and 200µl of CHCl3/methanol (2:1) were added to each sample (ventricles- 50µg protein in 10µl; plasma- 10µl) before being briefly vortexed. Samples were first mixed (rotary mixer, 10 min), sonicated (water bath, 30 min at room temperature [RT]) then rested at RT (20 min). Samples were centrifuged (16,000 g, 10 min) and the supernatant was dried under a constant stream of nitrogen gas at 40°C. Extracted lipids were resuspended in 50µl H2O saturated butanol then sonicated (10 min), before 50 µl of methanol with 10mM ammonium was added. Resuspended extracts were centrifuged (3,350 g, 5 min), and the supernatant transferred to glass vials with Teflon insert caps and stored at -80°C until analyzed. Lipid extracts were thawed at RT for an hour, then sonicated in a water bath for 15 min at RT before analysis. |