Summary of Study ST001101
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000737. The data can be accessed directly via it's Project DOI: 10.21228/M8DM5Q This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
Study ID | ST001101 |
Study Title | Identification of potential sepsis biomarkers in burn injury conditions. |
Study Summary | 12 mice were subjected to one of four treatment groups: no burn injury:no injection, burn injury:no infection, no burn injury:infection, and burn injury:infection. The researchers in this study are looking for metabolites in serum that could indicate sepsis in mice with burn injuries. |
Institute | Texas Tech University |
Department | Department of Biological Sciences |
Last Name | Elmassry |
First Name | Moamen |
Address | 2901 Main St, Lubbock, TX 79409 |
moamen.elmassry@ttu.edu | |
Phone | 806-742-2715 |
Submit Date | 2018-11-29 |
Num Groups | 4 |
Total Subjects | 12 |
Raw Data Available | Yes |
Analysis Type Detail | GC-MS |
Release Date | 2019-01-22 |
Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Sample Preparation:
Sampleprep ID: | SP001152 |
Sampleprep Summary: | 30µL of plasma was aliquoted into a 1.5 mL eppendorph tube. 1 mL of 3:3:2 ACN:H20:IPA was added to each sample. Samples were vortexed for 10 seconds and then shaken at 4°C for 5 minutes using an orbital mixing plate. Samples were centrifuged for 2 minutes at 14,000 rcf. 2 x 475 µL aliquots were removed, one dried down and submitted to derivitization, the other frozen at -20°C for backup. Samples were derivatized by adding 10 µL of MeOX and then shaken at 30°C for 1.5 hours using a orbital mixing plate. 91 µL of MSTFA + FAMES mixutre is added to each sample. Samples were shaken at 37°C for 30 minutes. Samples submitted to GC analysis. |