Summary of Study ST001141
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000762. The data can be accessed directly via it's Project DOI: 10.21228/M86103 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
Study ID | ST001141 |
Study Title | Evaluation of metabolome sample preparation and extraction methodologies for oleaginous filamentous fungi Mortierella alpina |
Study Type | method optimization |
Study Summary | In this study, based on the method of fast filtration, we evaluated the three metabolomics analysis protocols commonly used for microbial metabolomics analysis in M. alpina and systematically optimised the metabolite extraction solvent. |
Institute | Jiangnan University |
Department | School of Food Science and Technology |
Laboratory | State Key Laboratory of Food Science and Technology |
Last Name | Hengqian |
First Name | Lu |
Address | 1800 Lihu Ave, Wuxi, Jiangsu 214122, P.R. China. |
hengqianlu@163.com | |
Phone | +86 15006176136 |
Submit Date | 2019-02-24 |
Analysis Type Detail | GC-MS |
Release Date | 2019-09-23 |
Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Sample Preparation:
Sampleprep ID: | SP001213 |
Sampleprep Summary: | The obtained supernatants were vacuum dried at 30°C prior to derivatisation. The extraction solution served as a negative control to subtract from the background spectrum. The dried extract was derivatised. Briefly, the vacuum dried samples were resuspended in 100 μL of MeOX (10 mg/mL) in pyridine and incubated at 37°C for 90 min in a block heater. Then, 40 μL of MSTFA+1%TMCS was pipetted into each sample and incubated at 37°C for another 30 min. The resulting solution from each sample was collected and transferred into gas chromatography (GC) vials for analysis. |