Summary of Study ST001241
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000830. The data can be accessed directly via it's Project DOI: 10.21228/M8D68Q This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
Study ID | ST001241 |
Study Title | Global Metabolic Analysis Trisomy 21 - Cohort 3, Plasma |
Study Summary | Global metabolic analysis of plasma from individuals with and without trisomy 21. |
Institute | University of Colorado Denver |
Last Name | Culp-Hill |
First Name | Rachel |
Address | 12801 E 17th Ave L18-9403D, Aurora, Colorado, 80045, USA |
rachel.hill@cuanschutz.edu | |
Phone | 303-724-5798 |
Submit Date | 2019-08-13 |
Raw Data Available | Yes |
Raw Data File Type(s) | raw(Thermo) |
Analysis Type Detail | LC-MS |
Release Date | 2019-08-21 |
Release Version | 1 |
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Sample Preparation:
Sampleprep ID: | SP001317 |
Sampleprep Summary: | For plasma analyses, a volume of 20μL of was extracted in 480μL of ice-cold methanol:acetonitrile:water (5:3:2). For CSF analyses, a volume of 20μL was extracted in 180μL and for cell-based experiments, 2 million cells were extracted in 1mL of the same ice-cold lysis solution. Subsequently, these solutions were vortexed for 30 minutes at 4°C. Insoluble proteins were pelleted by centrifugation (10 minutes at 4°C and 12,000 g) and supernatants were collected and stored at -80°C until analysis. For quantitative analysis of kynurenine pathway (KP) metabolites, supernatants were spun in a Speedvac until dry and resuspended in 0.1% formic acid in water as previously described (PMID: 30213797, 30143553). |