Summary of Study ST001344
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000914. The data can be accessed directly via it's Project DOI: 10.21228/M8JH69 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
Study ID | ST001344 |
Study Title | C56BL6 WT or MyD88KO BMDM stimulated with different TLRs (part-IV) |
Study Type | WT vs MyD88 deficient macrophages |
Study Summary | WT control or MyD88 deficient bone marrow derived macrophages were stimulated with TLR2, TLR3, TLR4, TLR7, and TLR9 ligands for 48 h. |
Institute | University of California, Los Angeles |
Last Name | Bensinger |
First Name | Steven |
Address | University of California, Los Angeles |
bensingerlab@gmail.com | |
Phone | 3102061016 |
Submit Date | 2020-04-01 |
Raw Data Available | Yes |
Raw Data File Type(s) | mzML |
Analysis Type Detail | MS(Dir. Inf.) |
Release Date | 2020-07-07 |
Release Version | 1 |
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Sample Preparation:
Sampleprep ID: | SP001426 |
Sampleprep Summary: | A modified Bligh and Dyer extraction (Bligh and Dyer, 1959) was carried out on samples. Prior to biphasic extraction, a 13-lipid class Lipidyzer Internal Standard Mix is added to each sample (AB Sciex, 5040156). Following two successive extractions, pooled organic layers were dried down in a Genevac EZ-2 Elite. Lipid samples were resuspended in 1:1 methanol/dichloromethane with 10 mM Ammonium Acetate and transferred to robovials (Thermo 10800107) for analysis. |