Summary of Study ST001350
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000920. The data can be accessed directly via it's Project DOI: 10.21228/M8S11R This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
Study ID | ST001350 |
Study Title | Extraction of high-resolution Metabolomics data of the Yeast Metabolic Cycle |
Study Summary | Extraction of a high resolution metabolomics dataset through the Yeast Metabolic Cycle to complete a multi-omics dataset in this model system |
Institute | University of Florida |
Last Name | Conesa |
First Name | Ana |
Address | 2033 Mowry Rd, Gainesville, FL 32610-3633 |
salvacasani@gmail.com / aconesa@ufl.edu | |
Phone | 352-273-8127 |
Submit Date | 2020-03-06 |
Raw Data Available | Yes |
Raw Data File Type(s) | raw(Thermo) |
Analysis Type Detail | LC-MS |
Release Date | 2020-12-01 |
Release Version | 1 |
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Sample Preparation:
Sampleprep ID: | SP001432 |
Sampleprep Summary: | Cell pellets were lyophilized to dryness overnight. The lyophilized pellet was homogenized in 50:50 acetonitrile/water with 0.3 % formic acid for OAs and AAs, 5% TCA for acetyl and malonyl CoA, 0.5M PCA for oxidized nucleotides and 50:50 methanol/NaOH for reduced nucleotides using the Precellys bead-based homogenizing system. Precellys tough micro-organism lysing kit (VK-05) was used to burst the yeast cells. An aliquot of homogenate for the required assay was aliquoted and immediately stored at -80oC. |