Summary of Study ST001361
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000931. The data can be accessed directly via it's Project DOI: 10.21228/M8BT4S This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
Study ID | ST001361 |
Study Title | Serum tryptophan metabolomics in CKD |
Study Type | MS quantitative analysis |
Study Summary | Serum was processed using a targeted metabolomics platform for quantifying tryptophan metabolites as a number of these metabolites are well establish uremic toxins. |
Institute | University of Florida |
Last Name | Ryan |
First Name | Terence |
Address | 1864 Stadium Rd, FLG 114, Gainesville, FL, 32611, USA |
ryant@ufl.edu | |
Phone | 352-294-1700 |
Submit Date | 2020-04-02 |
Num Groups | 2 |
Total Subjects | 16 |
Num Males | 8 |
Num Females | 8 |
Raw Data Available | Yes |
Analysis Type Detail | LC-MS |
Release Date | 2020-12-31 |
Release Version | 1 |
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Sample Preparation:
Sampleprep ID: | SP001443 |
Sampleprep Summary: | Twenty-five microliters of each mouse serum was spiked with 5 µL internal standards (IS) solution consisted of tryptophan ¹³C₁₁, serotonin D4, kynurenine D4, kynurenic Acid D5, xanthurenic acid D4, anthranilic acid ¹³C₆, indoxyl sulfate ¹³C₆, p-cresol sulfate D7 and 3-indole-acetate D7. Metabolite extraction was done by protein precipitation using 200 µl of 8:1:1 Acetonitrile: Methanol: Acetone with 0.1% formic acid. Further protein precipitation was allowed by incubating the samples at 4°C for 20 min. Samples were placed in an ultrasonic bath for 10 min and then centrifuged at 20 000 xg for 5min at 4°C to pellet the protein. 190 µl supernatant was transferred from each sample into clean tube and dried under a gentle stream of nitrogen at 30°C. The dried extracts were re-suspended with 25 µL water with 0.1% formic acid. Resuspension was allowed at 4°C for 10 -15 min then samples were centrifuged at 20000 xg for 5 min at 4°C. Supernatants were transferred into clean LC-vials for targeted LC-MS quantitation on a Thermo Q-Exactive Oribtrap mass spectrometer with Dionex UHPLC and autosampler. |