Summary of Study ST001412
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000968. The data can be accessed directly via it's Project DOI: 10.21228/M8K688 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
Study ID | ST001412 |
Study Title | Metabolomics study in Plasma of Obese Patients with Neuropathy Identifies Potential Metabolomics Signatures |
Study Summary | The goal of this study was to characterize biochemical profiles observed in human plasma samples originating from an obese cohort stratified by a diagnosis of neuropathy as well as a cohort of lean control subjects without a clinical manifestation of neuropathy. |
Institute | University of Michigan |
Last Name | Feldman |
First Name | Eva |
Address | 5017 AATBSRB, 109 Zina Pitcher Place Ann Arbor, MI 48109-2200 |
efeldman@med.umich.edu | |
Phone | 734-763-7274 |
Submit Date | 2020-06-22 |
Raw Data File Type(s) | wiff |
Analysis Type Detail | LC-MS |
Release Date | 2021-06-15 |
Release Version | 1 |
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Sample Preparation:
Sampleprep ID: | SP001494 |
Sampleprep Summary: | Samples were prepared using the automated MicroLab STARĀ® system from Hamilton Company. Several recovery standards were added prior to the first step in the extraction process for QC purposes. To remove protein, dissociate small molecules bound to protein or trapped in the precipitated protein matrix, and to recover chemically diverse metabolites, proteins were precipitated with methanol under vigorous shaking for 2 min (Glen Mills GenoGrinder 2000) followed by centrifugation. The resulting extract was divided into five fractions: two for analysis by two separate reverse phase (RP)/UPLC-MS/MS methods with positive ion mode electrospray ionization (ESI), one for analysis by RP/UPLC-MS/MS with negative ion mode ESI, one for analysis by HILIC/UPLC-MS/MS with negative ion mode ESI, and one sample was reserved for backup. Samples were placed briefly on a TurboVapĀ® (Zymark) to remove the organic solvent. The sample extracts were stored overnight under nitrogen before preparation for analysis. |