Summary of Study ST001610
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001035. The data can be accessed directly via it's Project DOI: 10.21228/M8X39Q This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
Study ID | ST001610 |
Study Title | Control (DMSO 0.1%; v/v) and 10 µM DRB18 treated A549 lung cancer cells in vitro for 48 hours |
Study Type | Anticancer compound treatment experiment |
Study Summary | Control (DMSO 0.1%; v/v) and 10 µM DRB18 were used to treated 5 million A549 lung cancer cells in vitro for 48 hours. The untargeted metabolomics analysis was performed on the cell lysates. The main objective of the study was to determine changes in metabolite abundances in lung cancer after treatment with DRB18, an inhibitor of glucose transporter proteins. |
Institute | Ohio University |
Department | Biological Sciences |
Laboratory | Dr. Xiaozhuo Chen, Edison biotechnology Institute |
Last Name | Shriwas |
First Name | Pratik |
Address | 172 Water Tower, Building 25, The Ridges, Konnekar Research Centerm Athens Ohio - 45701, USA |
ps774614@ohio.edu | |
Phone | 740-603-3801 |
Submit Date | 2020-11-08 |
Num Groups | 2 |
Raw Data Available | Yes |
Raw Data File Type(s) | d |
Analysis Type Detail | LC-MS |
Release Date | 2020-12-09 |
Release Version | 1 |
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Sample Preparation:
Sampleprep ID: | SP001693 |
Sampleprep Summary: | 5 × 106 A549 cells were treated with or without DRB18 for 48 hours. After treatment, cells were washed twice with deionized water and polar metabolites were then extracted with cryogenically cold 80% methanol/water mixture. LC-MS grade water, methanol, and acetonitrile (Fischer Scientific, PA, USA) were used. Methanol-extracted samples were then sonicated in cycles of sonication phase and rest phase for 10 minutes (5 second sonication phase and 10 seconds halt). The samples were then centrifuged at 13,000 rpm for 10 minutes and supernatant was then collected. Supernatants collected from in vitro and in vivo extraction were then lyophilized. Briefly, the supernatant was then lyophilized by using a speed vaccum evaporator. The samples were then dissolved into a mixture of acetonitrile/water (1:1; v/v). |
Processing Method: | Quenching |
Processing Storage Conditions: | -80℃ |
Extraction Method: | Quenching with Ice cold methanol |
Extract Enrichment: | Speed vaccum evaporator |
Extract Storage: | -80℃ |
Sample Resuspension: | Acetonitrile/waster (1:1) |