Summary of Study ST001614
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001037. The data can be accessed directly via it's Project DOI: 10.21228/M8NM4H This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
Study ID | ST001614 |
Study Title | NMR metabolomics analysis of ricin-induced and fasting hypoglycemia (part-I) |
Study Type | Targeted NMR |
Study Summary | Mice were subject to ricin exposure or fasting conditions for 2 hours, 8 hours, or an overnight time period. Following treatment, livers were removed and metabolites were extracted and analyzed by NMR. |
Institute | Montana State University |
Department | Chemistry & Biochemistry |
Laboratory | Copié |
Last Name | Kempa |
First Name | Jake |
Address | 103 Chemistry and Biochemistry Building |
jkempa97@gmail.com | |
Phone | 4067997200 |
Submit Date | 2020-09-23 |
Num Groups | 9 |
Total Subjects | 107 |
Num Males | 54 |
Num Females | 53 |
Raw Data Available | Yes |
Raw Data File Type(s) | d |
Analysis Type Detail | NMR |
Release Date | 2022-11-29 |
Release Version | 1 |
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Sample Preparation:
Sampleprep ID: | SP001697 |
Sampleprep Summary: | Frozen livers were homogenized in liquid nitrogen using a mortar and pestle. Liver powder was weighed (60-80 mg) into 2-mL microcentrifuge tubes. 900 µL of ice-cold 1:2 MeOH/CHCl3 was added to the liver tissue and tissue was mixed into suspension and lysed using a FastPrep-24TM 5G homogenizer using the S. aureus setting of 6.0 meters/second speed for 2 x 40 seconds. Tubes were placed back on ice and 200 µL of ice-cold H2O was added, before repeating the lysis step. Samples were then placed at -20 ºC for approximately 45 minutes to precipitate protein, then centrifuged at 14,000 g for 10 minutes. 250 µL of upper polar phase were transferred to a 1.5 mL Eppendorf tube and dried overnight using a Savant™ Universal SpeedVac™ vacuum system with no heat. Dried metabolite mixtures were re-suspended in 600 µL of NMR buffer containing 0.25 mM 4,4-dimethyl-4-silapentane-1-sulfonic acid (DSS), 90%H2O/10% D2O, 25 mM sodium phosphate, pH 7. |