Summary of Study ST001642
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001050. The data can be accessed directly via it's Project DOI: 10.21228/M8ZX18 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
Study ID | ST001642 |
Study Title | Lipidomics in high-risk subjects for the identification of integrated biomarker signatures of type 1 diabetes |
Study Summary | We present the lipidome of plasma collected from high-risk type 1 diabetes subjects. The methyl tert-butyl ether (MTBE) method was used for lipid extraction, followed by high performance liquid chromatography (HPLC) tandem mass spectrometry (LC-MS/MS) using a Q Exactive Orbitrap mass spectrometer and an Accela 600 HPLC. Lipid species were identified and quantified by analyzing the raw files in LipidSearch 4.2. Further analysis was conducted using Graphpad Prism and Ingenuity Pathway Analysis (IPA). |
Institute | University of Miami |
Last Name | Bhattacharya |
First Name | Sanjoy |
Address | 1638 NW 10th Avenue, Room 706-A, Miami, FL 33136 |
sbhattacharya@med.miami.edu | |
Phone | 305-482-4103 |
Submit Date | 2021-01-06 |
Raw Data Available | Yes |
Raw Data File Type(s) | raw(Thermo) |
Analysis Type Detail | LC-MS |
Release Date | 2021-01-25 |
Release Version | 1 |
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Sample Preparation:
Sampleprep ID: | SP001725 |
Sampleprep Summary: | Lipids were extracted from 50 µL of plasma by adding 4 mL of methyl tert-butyl ether (MTBE) and 1.2 mL of butylated hydroxytoluene (BHT) then incubating overnight at 4°C. The following day, 1.25 mL of 0.15 M ammonium acetate was added, and the samples were centrifuged at 2,000 x g for 10 minutes at 4°C to obtain phase separation. The upper organic phase was collected and the extracted lipids equivalent to 100 µg of protein were dried in a speed vacuum concentrator. The samples were reconstituted in 50 µL of chloroform:methanol (1:1) and sonicated before analysis by liquid chromatography tandem mass spectrometry (LC-MS/MS). The lipids were analyzed by LC-MS/MS using an Accela 600 HPLC and a Q Exactive Orbitrap mass spectrometer. |