Summary of Study ST001652
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001058. The data can be accessed directly via it's Project DOI: 10.21228/M8XX2B This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
Study ID | ST001652 |
Study Title | Atypical Molecular Basis for Drug Resistance to Mitochondrial AQ: A Function Inhibitors in Plasmodium falciparum |
Study Summary | In this study, we present a clear genotype for the P. falciparum SB1-A6 acridone-resistant clonal parasite strain and, through a combination of targeted and whole-cell methods, establish that the mechanism of resistance to both cytochrome bc1 and DHODH inhibitors results from the contribution of multiple genetic polymorphisms. We find that P. falciparum SB1-A6 accumulates both a copy number variation and a specific mutation in PfDHODH, and both of these genetic polymorphisms contribute to the panresistant phenotype. This study uncovers a mechanism of cross-resistance between PfDHODH and mtETC inhibitors and serves as a cautionary note to future antimalarial combination therapy formulations containing such drugs. |
Institute | U.S. Food & Drug Administration |
Last Name | Painter |
First Name | Heather |
Address | 10903 New Hampshire Ave., WO 52/72-5324, Silver Spring, MD 20993 |
Heather.Painter@fda.hhs.gov | |
Phone | 240-402-2040 |
Submit Date | 2021-01-17 |
Publications | DOI:10.1128/AAC.02143-20 |
Raw Data Available | Yes |
Raw Data File Type(s) | mzXML |
Analysis Type Detail | LC-MS |
Release Date | 2021-02-01 |
Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Sample Preparation:
Sampleprep ID: | SP001735 |
Sampleprep Summary: | 90% Methanol/10% water at 50:1 solvent/cell pellet followed by vortexing and clarification via centrifugation |
Processing Method: | Lysis |
Processing Storage Conditions: | 4℃ |
Extract Cleanup: | Clarification via centrifugation followed by nitrogen drying |
Extract Storage: | -80℃ |
Sample Resuspension: | 6X pellet volume of HPLC H2O |
Sample Spiking: | Internal Standard 13C4, 15N1-Aspartate used for sample preparation normalizer |