Summary of Study ST001712
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001096. The data can be accessed directly via it's Project DOI: 10.21228/M81M6P This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
Study ID | ST001712 |
Study Title | Metabolomics analysis of plasma from a mouse model of astrocytoma subjected to radiotherapy |
Study Summary | Mice were randomized in two groups (n=9 mice/group), one group was subjected to radiotherapy (Monday and Friday for 2 consecutive weeks at 3Gy/session) and the other cohort was the control. Sample were taken approximately each 10 days from the tail vein. |
Institute | National Cancer Institute |
Last Name | Larion |
First Name | Mioara |
Address | 37 Convent Dr, Building 37 Room 1136 |
mioara.larion@nih.gov | |
Phone | 2407606825 |
Submit Date | 2021-02-26 |
Analysis Type Detail | LC-MS |
Release Date | 2021-03-09 |
Release Version | 1 |
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Sample Preparation:
Sampleprep ID: | SP001795 |
Sampleprep Summary: | Blood samples were separated into plasma and packed cells by centrifugation at 3,500g for 15 min at 4°C and stored at -80°C until extraction. 35 µL of plasma were extracted in a water:methanol:chloroform mixture. Centrifuged for 20 min at 4°ᵒC and 13,000 rpm and the resulting upper hydrophilic phase was then transferred to a clean vial and dried under a stream of N2 gas. Dried sediments were resuspended in 60% methanol (aq.) and injected into the LCMS system. |