Summary of Study ST001780
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001133. The data can be accessed directly via it's Project DOI: 10.21228/M87X24 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
Study ID | ST001780 |
Study Title | Comparative analysis of metabolomic profiles in cerebrospinal fluid before and after endurance exercise |
Study Summary | This study compared the metabolomic differences in the cerebrospinal fluid of young adult volunteers between before (Pre), and 60 minutes after the 90-min run (Post). |
Institute | University of California, San Diego School of Medicine |
Last Name | Naviaux |
First Name | Robert |
Address | 214 Dickinson St., Room C-107, San Diego, CA, 92103, USA |
rnaviaux@health.ucsd.edu | |
Phone | 619-543-2904 |
Submit Date | 2021-05-03 |
Num Groups | 2 |
Total Subjects | 19 |
Num Males | 13 |
Num Females | 6 |
Analysis Type Detail | LC-MS |
Release Date | 2021-11-25 |
Release Version | 1 |
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Sample Preparation:
Sampleprep ID: | SP001863 |
Sampleprep Summary: | The cerebrospinal fluid samples (90 µl) were mixed with 5 µl of commercial internal standards and 5 µl of custom-synthesized 13C labeled standards and then incubated for 10 min at room temperature to permit small molecules and vitamins in the internal standards to associate with binding proteins. The metabolites were extracted with 400 µl of cold (-20 °C) methanol: acetonitrile (50:50, v/v), yielding a final concentration of methanol: acetonitrile: water (40:40:20, v/v/v). The samples were then vortexed vigorously, incubated on crushed ice for 10 min, and then centrifuged at 16,000 g for 10 min at 4 °C. The supernatants containing the extracted metabolites and internal standards were transferred to labeled cryotubes and stored at -80 °C prior to LC-MS/MS. |
Processing Storage Conditions: | On ice |
Extract Cleanup: | None |
Extract Storage: | -80℃ |
Sample Resuspension: | None |
Sample Derivatization: | None |