Summary of Study ST001785

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001134. The data can be accessed directly via it's Project DOI: 10.21228/M8470J This work is supported by NIH grant, U2C- DK119886.

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Study ID	ST001785
Study Title	The human lipidome via NMR
Study Summary	The study population includes 844 healthy volunteers of which 183 women and 661 men, with a median age of 43 ± 12 yrs and 40 ± 11 yrs, respectively. The participants in this study were selected from the Tus-cany section of the Italian Association of Blood Donors (AVIS) in the Transfusion Service of the Pistoia Hospital. Plasma samples were obtained according to the Italian guidelines for blood donations. How age and sex influence the human metabolome and lipidome were investigated.
Institute	University of Florence
Department	CERM
Last Name	Vignoli
First Name	Alessia
Address	Via Luigi Sacconi 6
Email	vignoli@cerm.unifi.it
Phone	0554574281
Submit Date	2021-04-26
Analysis Type Detail	NMR
Release Date	2021-05-25
Release Version	1

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Sample Preparation:

Sampleprep ID:	SP001868
Sampleprep Summary:	Frozen plasma samples were thawed at room temperature and shaken before use (Bernini et al. 2011). A total of 300 μL of a sodium phosphate buffer (10.05 g Na2HPO4·7H2O; 0.2 g NaN3; 0.4 g sodium trimethylsilyl [2,2,3,3-2H4] pro-pionate (TMSP) in 500 mL of H2O with 20% (v/v) 2H2O; pH 7.4) was added to 300 μL of each plasma sample, and the mixture was homogenized by vortexing for 30 seconds. A total of 450 μL of this mixture was transferred to a 4.25 mm NMR tube (Bruker BioSpin srl) for analysis.

Sampleprep ID:

SP001868

Sampleprep Summary:

Frozen plasma samples were thawed at room temperature and shaken before use (Bernini et al. 2011). A total of 300 μL of a sodium phosphate buffer (10.05 g Na2HPO4·7H2O; 0.2 g NaN3; 0.4 g sodium trimethylsilyl [2,2,3,3-2H4] pro-pionate (TMSP) in 500 mL of H2O with 20% (v/v) 2H2O; pH 7.4) was added to 300 μL of each plasma sample, and the mixture was homogenized by vortexing for 30 seconds. A total of 450 μL of this mixture was transferred to a 4.25 mm NMR tube (Bruker BioSpin srl) for analysis.