Summary of Study ST001795
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001139. The data can be accessed directly via it's Project DOI: 10.21228/M8GH59 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
Study ID | ST001795 |
Study Title | Changes in mesenteric lymph lipid profile of mice upon high-fat diet with and without Celecoxib (part I) |
Study Type | Untargeted lipidomics analysis |
Study Summary | Untargeted lipid profiling of mesenteric mice lymph from mice fed with CHOW, HFD and HFD supplemented with COx-2 inhibitor drug Celecoxib. It is proposed that Celecoxib can protect from deleterious morphological changes in lymphatic system caused by obesity/HFD. |
Institute | Monash Institute of Pharmaceutical Sciences |
Department | Drug Delivery, Disposition and Dynamics |
Laboratory | Trevaskis Lab, Creek Lab |
Last Name | Anderson |
First Name | Dovile |
Address | 6 Anderson |
dovile.anderson@gmail.com | |
Phone | 8671141 |
Submit Date | 2021-05-12 |
Num Groups | 3 |
Total Subjects | 26 |
Num Males | 26 |
Publications | Manuscript NATMETAB-A20022406A Mesenteric lymphatic dysfunction promotes insulin resistance and represents a potential novel treatment target in obesity |
Raw Data Available | Yes |
Raw Data File Type(s) | raw(Thermo) |
Analysis Type Detail | LC-MS |
Release Date | 2021-06-02 |
Release Version | 1 |
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Sample Preparation:
Sampleprep ID: | SP001878 |
Sampleprep Summary: | For lipidomics analysis, lipid was extracted from mesenteric lymph by chloroform:methanol (1:3). Samples were vortexed for 1 h at 4°C and then centrifuged at 16,000 g for 10 min. Supernatant was carefully transferred to another tube and stored at -80°C until analysis. Before LC-MS analysis, the extract was dried with nitrogen and reconstituted in 20 µl water and 180 µl butanol-methanol (1:1 v/v). The reconstituted extract was vortexed (Vortex mixer, Ratek) for 200 sec with 20 cycles of 5 sec spin and 20 sec vortex. The extract was sonicated in a water bath for 1 hour which was maintained at <20°C by sonicating the samples on ice. The samples were then centrifuged for 10 min at 16,000 g and the supernatant was transferred to LC-MS vials and stored at 4°C prior to analysis. |
Processing Storage Conditions: | 4℃ |
Extract Storage: | -80℃ |