Summary of Study ST001876
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001182. The data can be accessed directly via it's Project DOI: 10.21228/M8XD6P This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
Study ID | ST001876 |
Study Title | Metabolomics analysis of multiple samples on Agilent 6546-Part 2 |
Study Type | Metabolomics |
Study Summary | Metabolomics analysis of multiple samples from mouse, trying to annotate the metabolites in them. Agilent 6546 LC-QTOF was used for metabolomics detection. |
Institute | Dalian Institute Of Chemical Physics |
Laboratory | Laboratory of High Resolution Separation/Analysis and Metabonomics |
Last Name | Zheng |
First Name | Fujian |
Address | No. 457 Zhongshan Road, Shahekou District, Dalian, Liaoning Province, China |
zhengfj@dicp.ac.cn | |
Phone | 18698730176 |
Submit Date | 2021-06-28 |
Raw Data Available | Yes |
Raw Data File Type(s) | d |
Analysis Type Detail | LC-MS |
Release Date | 2021-07-24 |
Release Version | 1 |
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Sample Preparation:
Sampleprep ID: | SP001959 |
Sampleprep Summary: | Faecal samples were collected and immediately stored at -80 °C for further analysis. After the mice were sacrificed, liver tissue was sampled, immediately frozen in liquid nitrogen, and then stored at -80 °C. The study was approved by the Animal Ethics Committee of Saiye Biology (ACU20-A027). Mouse liver tissue (20 mg) and 600 μL CH3OH/H2O (8:2, vol/vol) solvent were combined in a centrifuge tube and then homogenized (28 Hz, 60 s and 30 s) in a frozen mixed ball grinding machine (MM400, Retsch Technology, Han, Germany). The mixture was centrifuged for 10 min at 15,000 g and 4 °C, 500 μL of supernatant was transferred to a new centrifuge tube for lyophilization, and the residue was reconstituted with 100 μL of Mix-IS-MeOH. Mouse faeces: A 60 mg sample of mouse faeces and 600 μL CH3OH/H2O (8:2, vol/vol) solvent were combined in a centrifuge tube and then homogenized (28 Hz, 60 s and 30 s) in a frozen mixed ball grinding machine (MM400, Retsch Technology, Han, Germany). The mixture was centrifuged for 10 min at 15,000 g and 4 °C; then, 500 μL of supernatant was transferred to a new centrifuge tube for lyophilization, and the residue was reconstituted with 100 μL of Mix-IS-MeOH. |