Summary of Study ST002001
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001269. The data can be accessed directly via it's Project DOI: 10.21228/M8PD9N This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
Study ID | ST002001 |
Study Title | A case-control study on plasma metabolomics analysis in Myalgic encephalomyelitis/chronic fatigue syndrome (ME/CFS) (Part 2) |
Study Summary | Targeted and untargeted metabolomics analysis consisting of 888 metabolic analytes covering primary metabolites, biogenic amines, complex lipids, and oxylipins in 106 ME/CFS cases and 91 frequency-matched healthy controls |
Institute | Columbia University |
Department | Center for Infection and Immunity |
Laboratory | Center for Infection and Immunity |
Last Name | Lipkin |
First Name | W. Ian |
Address | 722 W. 168th St., 17th Floor, New York, NY, 10032 |
wil2001@cumc.columbia.edu | |
Phone | 212-342-9033 |
Submit Date | 2021-11-22 |
Raw Data Available | Yes |
Analysis Type Detail | LC-MS |
Release Date | 2021-12-08 |
Release Version | 1 |
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Sample Preparation:
Sampleprep ID: | SP002088 |
Sampleprep Summary: | 1 Thaw plasma samples on ice 2 Vortex plasma sample and pipette 50µl in polypropylene Eppendorf tube/Plate 3 Add Surrogate Standards of the 3 lipid classes (as needed) and other reagents as in the following table: Plasma Sample 10x Oxylipid Surrogate STDs CUDA + PHAU Anti-oxidant ACN/MeOH SUM 4 Vortex for 30sec 5 Centrifuge at 6°C for 5 min at 15,000 g (or 1,800 g for plates) 6 Take the supernatant (~240µl) into new Eppendorf tube/filter plate 7 Spin Filter (0.1 um) or plate filter (0.2um) for 5 min @ 12,000 g (1,800g for plate) 8 place aliquotes of samples into vials, or plate, and cap vials or seal plates 9 Store in -20°C until analysis |