Summary of Study ST002011
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001275. The data can be accessed directly via it's Project DOI: 10.21228/M8WX21 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
Study ID | ST002011 |
Study Title | The anticancer human mTOR inhibitor MLN0128/Sapanisertib with potent multistage in vitro antiplasmodium activity and in vivo antimalarial efficacy in a humanised mouse model is an inhibitor of multiple Plasmodium falciparum kinases. |
Study Summary | Here we interrogated the in vitro metabolic effects of 6 drugs using ultra-high performance liquid chromatography mass-spectrometry (UHPLC-MS). The resulting metabolic fingerprints provide information on the parasite biochemical pathways affected by pharmacologic intervention and offer a critical blueprint for selecting and advancing lead compounds as next-generation antimalarial drugs. Our results reveal several distinctions between compounds with polypharmacological effects. |
Institute | Pennsylvania State University |
Department | Department of Biochemistry and Molecular Biology |
Last Name | Llinas |
First Name | Manuel |
Address | W126 Millenium Science Complex, University Park, PA 16802 |
manuel@psu.edu | |
Phone | 814-867-3527 |
Submit Date | 2021-12-08 |
Raw Data Available | Yes |
Analysis Type Detail | LC-MS |
Release Date | 2022-11-02 |
Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Sample Preparation:
Sampleprep ID: | SP002098 |
Sampleprep Summary: | 90% Methanol/10% water at 50:1 solvent/cell pellet followed by vortexing and clarification via centrifugation. |
Processing Method: | Lysis |
Processing Storage Conditions: | 4? |
Extraction Method: | See SA Cobbold et al JBC 2013 |
Extract Cleanup: | Clarification via centrifugation followed by nitrogen drying |
Extract Storage: | -80? |
Sample Spiking: | Internal Standard 13C4, 15N1-Aspartate used for sample preparation normalizer |