Summary of Study ST002369
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001523. The data can be accessed directly via it's Project DOI: 10.21228/M8TQ5G This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
Study ID | ST002369 |
Study Title | The impact of acute Colony Stimulating Factor 1 treatment on serum and liver metabolites in fed and fasted mice LIVER |
Study Type | Drug treatment |
Study Summary | The aim of the study was to investigate the impact of expanding tissue macrophage populations on systemic metabolism. Groups of male mice were treated with 4 x daily injections of Colony Stimulating Factor (1), followed by normal feeding or a 24 h fast from day 6 to day 7. Serum and liver were collected for GC-MS metabolomic analysis on a Shimadzu TQ8050. |
Institute | University of Queensland |
Department | Mater Research-UQ |
Laboratory | Irvine |
Last Name | Irvine |
First Name | Katharine |
Address | Translational Research Institute, 37 Kent St |
katharine.irvine@uq.edu.au | |
Phone | +61734437655 |
Submit Date | 2022-11-27 |
Num Groups | 4 |
Total Subjects | 20 |
Num Males | 20 |
Raw Data Available | Yes |
Raw Data File Type(s) | mzXML |
Analysis Type Detail | GC-MS |
Release Date | 2023-08-01 |
Release Version | 1 |
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Sample Preparation:
Sampleprep ID: | SP002464 |
Sampleprep Summary: | Metabolites were extracted from 50 µl serum using 150 µl methanol and 50 µl chloroform, including 13C-sorbitol and 13C,15N-valine internal standards, and 30 µl of clarified sera was prepared for GC-MS analysis. Liver metabolites were extracted from 20-40 mg liver tissue (sampled from the same region of the left lateral lobe) in 600 µl methanol:MilliQ water (3:1), including 13C-sorbitol and 13C,15N-valine internal standards. The tissue was cryomilled at 4°C. 110 µl chloroform was added to 440 µg homogenate which was transferred to a new tube and the sample thermomixed for 20 minutes then centrifuged at 13,000 rpm for 15 minutes. 30 µl supernatant was dried in inserts for GC-MS analysis. Dried samples for targeted analysis were derivatised online using the Shimadzu AOC6000 autosampler robot. Derivatisation was achieved by the addition of 25 µL methoxyamine hydrochloride (30 mg/mL in pyridine, Merck) followed by shaking at 37°C for 2h. Samples were then derivatised with 25 µL of N,O-bis (trimethylsilyl)trifluoroacetamide with trimethylchlorosilane (BSTFA with 1% TMCS, Thermo Scientific) for 1h at 37°C. The sample was allowed to equilibrate at room temperature for 1 h before 1 µL was injected onto the GC column using a hot needle technique. Split (1:10) injections were performed for each sample. |