Summary of Study ST002375
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001528. The data can be accessed directly via it's Project DOI: 10.21228/M8612V This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
Study ID | ST002375 |
Study Title | Metabolomics analysis of WT or GOT1 knockout CD8+ T cells cultured in serine-replete or serine-free media |
Study Summary | WT or GOT1 knockout CD8+ T cells were activated with plate-bound anti-CD3 or soluble anti-CD28, in serine-replete or serine-free media for 24 hours. Intracellular metabolome were assessed by MS. |
Institute | Johns Hopkins University |
Last Name | Xu |
First Name | Wei |
Address | 1650 Orleans Street, Baltimore, MD 21287, USA. |
wxu29@jhmi.edu | |
Phone | 443-220-9936 |
Submit Date | 2022-11-30 |
Raw Data Available | Yes |
Raw Data File Type(s) | wiff |
Analysis Type Detail | LC-MS |
Release Date | 2023-11-28 |
Release Version | 1 |
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Sample Preparation:
Sampleprep ID: | SP002470 |
Sampleprep Summary: | Cell pellets were spun down and washed twice with pre-warmed PBS. Metabolites were immediately extracted by adding methanol:water (80:20, v/v) extraction solution, sonicated and stored at -80 °C for at least 2 hours to precipitate the proteins. Supernatant after centrifugation at 14,000xg for 10 minutes was dried under nitrogen gas. Metabolites were then reconstituted using ACN:water (50:50, v/v) overnight at 4 °C. Soluble metabolites after centrifugation at 14,000xg for 10 minutes were subjected to targeted metabolite analysis by liquid-chromatography tandem mass spectrometry (LC-MS/MS). |