Summary of Study ST002418
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001556. The data can be accessed directly via it's Project DOI: 10.21228/M8K429 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST002418 |
| Study Title | Serum metabolomics and lipidomics profiling in iliac arteriovenous fistula creation in clinical patients: part 2 |
| Study Type | Study of the serum metabolome in patients with end stage kidney disease before and 6-weeks after iliac arteriovenous fistula creation via 1H NMR |
| Study Summary | This project is focused on a longitudinal analysis of the small molecules metabolomes in end stage renal disease patients undergoing iliac arteriovenous fistula creation using solution state NMR spectroscopy. It was hypothesized that after 6-weeks of AVF creation, these patients would present with altered serum metabolite features. |
| Institute | University of Florida |
| Department | Applied Physiology and Kinesiology |
| Laboratory | Rm 42 and Rm 43 |
| Last Name | Khattri |
| First Name | Ram |
| Address | 1864 Stadium RD, Gainesville, FL, 32611, USA |
| rbk11@ufl.edu | |
| Phone | 3307856045 |
| Submit Date | 2022-12-22 |
| Num Groups | 2 |
| Total Subjects | 45 subjects, 2 samples per subject, total of 90 samples |
| Num Males | 36 |
| Num Females | 9 |
| Study Comments | Study of the serum metabolome in patients with end stage kidney disease before and 6-weeks after iliac arteriovenous fistula creation via 1H NMR |
| Raw Data Available | Yes |
| Raw Data File Type(s) | fid |
| Analysis Type Detail | NMR |
| Release Date | 2023-12-22 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Sample Preparation:
| Sampleprep ID: | SP002513 |
| Sampleprep Summary: | Serum samples are associated with binding of residual proteins with DSS signal, resulting broaden DSS peak. The presence of the residual proteins, even after performing modified FOLCH extraction, was observed for serum samples, making it un-reliable for quantitative purpose. In this study, we coupled two extraction protocols to extract metabolites from the serum samples. Modified FOLCH extraction was performed as a first step to separate out water-soluble and lipid-soluble metabolites/compounds. Furthermore, acetonitrile:isopropanol:water (3:3:2 vol/vol/vol) extraction was performed on aqueous phase samples that we obtained from modified FOLCH extraction. |
| Sampleprep Protocol Filename: | AVF-NMR_Lipid_Phase_Procedures.docx |
| Processing Method: | Lyophilization and Homogenization |
| Processing Storage Conditions: | -80℃ |
| Extraction Method: | Modified FOLCH extraction for lipid phase samples |
| Extract Storage: | -80℃ |
| Sample Resuspension: | In 700 microliter of CDCl3 with 10 mM of pyrazine as internal standard for lipid phase samples |
| Sample Spiking: | 10 mM of TSP for urine samples |
